Přehled

Doctoral study program: Life Sciences (Faculty of Science, Masaryk University)

Research area: Structural Biology

Supervisor: Radek Marek

Annotation

The project is focused on detailed structural characterization of short purine oligonucleotides clipped by proper sequential motifs that induce parallel orientation of DNA strands. For this purpose, NMR experiments combined with MD simulations will be employed. The effect of modifications of selected nucleotides on the structural properties of designed models will be investigated to gain deeper understanding of key interactions that contribute to the folding of such systems.

Recommended literature

• Aleš NOVOTNÝ, Jan NOVOTNÝ, Iva KEJNOVSKÁ, Michaela VORLÍČKOVÁ, Radovan FIALA and Radek MAREK. Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip. Nucleic Acids Research, 2021, 49, 11425. doi:10.1093/nar/gkab915.

Funding

RG budget, Czech Science Foundation

Requirements on candidates

Structural chemistry or biology, advanced NMR spectroscopy, computational chemistry

Keywords

NMR spectroscopy, DNA, base pairing, molecular modelling

Information on the supervisor

ResearcherID: D-6929-2012, currently 3 PhD students, 11 alumni

Information about the application process: https://www.ceitec.eu/ls-mm-phd/

Application webpage: https://www.ceitec.eu/structure-of-parallel-forms-of-nucleic-acids-nmr-spectroscopy-and-molecular-modeling/t11452