Overview

Viral proteases are crucial for successful viral replication. We will elucidate the details of their proteolytic autoactivation and search for protease-specific inhibitors. Our focus will mainly be on proteases from flaviviruses (Zika, Dengue), coronaviruses (SARS-CoV-2), and retroviruses (HIV).The project involves designing mutated protein variants, cloning, protein expression, and purification, along with screening compound libraries. These steps will be followed by biophysical characterization and crystallization. Additionally, we will focus on developing and adapting cell-based phenotypic assays and designing light-activatable protease variants.This work seeks to contribute to opening new paths to potentially innovative therapies.

University:

Faculty of Science, Charles University

Group:

Jan Konvalinka Group, Proteases of Human Pathogens

Tutor:

Taťána Majerová, Ph.D.

Field of study:

Biochemistry, Physical chemistry

How to apply:

At the Institute of Organic Chemistry and Biochemistry of the CAS / IOCB Prague, we are looking for talented, independent, and highly motivated PhD students with an MSc. degree or equivalent in life sciences or related fields. We offer exciting new projects in organic and medicinal chemistry, biochemistry, cell biology, molecular and structural biology, and analytical and physical chemistry.

• Online registration deadline: 3 March 2025
• Interview Day: 20 March 2025

Learn more and apply at www.uochb.cz/en/call-for-phd-applications.